In Vitro Diagnostics

Insights gained through the use of in vitro diagnostics have become increasingly important in recent years. It is now hard to imagine medicine without them. They are not only used to diagnose physiological and pathological conditions, but also support the monitoring of therapeutic measures.

Since 2016, we have expanded our product portfolio to include products for point-of-care determination using vitro diagnostics. Here too, we cooperate with renowned universities and institutions all over the world to make our contribution to diagnostics and therapy.

LISA-CHE® – Determination of cholinesterase activity

The activities of two enzymes, namely acetylcholinesterase (ACzhE) and butyrylcholinesterase (BChE), can serve as biomarkers for detecting various diseases at an early stage. Examples of application include:

    • detection of post-operative delirium
    • detection of systemic inflammation
    • assessment of severity of inflammatory disease
    • detection of poisoning with organophosphates
    • detection of Alzheimer’s disease
    • detection of protein deficiency
    • detection of protein deficiency

LISA-CHE® is a portable point-of-care measuring device for determination of acetylcholinesterase and butyrylcholinesterase in whole blood.

Only one drop of whole blood is needed for the measurement, and the results are available within 4 minutes.

Thus, LISA-CHE® is not only an appropriate tool for early detection of certain conditions, but also for the monitoring of therapy measures.

LISA-H – Direct determination of current heparin activity

Heparin has become a standard medicine in anticoagulation management due to its anticoagulant effect, rapid onset of action and the possibility of antagonization.

In cardiac surgery with extracorporeal circulation, for instance, heparin is administered in high doses to prevent the blood from clotting due to contact with the large foreign surface. However, there is currently no method available by which heparin levels can be measured directly during surgery.

To close this gap, Dr. Franz Köhler Chemie GmbH has developed a novel device: LISA-H. By combining the conventional measurement of clotting time and the measurement of the current heparin level, this device enables the desired heparin level to be maintained during surgery and also allows the dosage of protamine (which is administered at the end of the procedure to neutralize the heparin) to be precisely controlled for the first time.

The ability to measure the heparin level can thus improve current anticoagulation management, thereby reducing bleeding complications and ultimately increasing patient safety and lowering costs.

M.A.I.K – Rapid test for the detection of nerve agents, pesticides and other cholinesterase inhibitors

Organophosphates inhibit the enzyme acetylcholinesterase (AChE), which plays an important role in the transmission of nerve stimuli.

Organophosphates are used as pesticides in agriculture. If used or stored improperly, this may lead to poisoning and cause significant harm to the human body. According to the WHO, organophosphate-based pesticides in particular cause millions of severe poisonings every year, some of which are fatal.

An even more toxic group of organophosphates are nerve agents such as sarin, VX and Novichok, which pose a current and serious threat (use of sarin in Syria, attacks on Kim Jong Nam with VX and on Sergei Skripal and Alexei Nawalny with Novichok). Here, it becomes clear that rapid detection of skin contamination is essential for taking countermeasures.

In cooperation with the Institute of Pharmacology and Toxicology of the German Armed Forces, we have developed M.A.I.K (Mobile Acetylcholinesterase Inhibitor Test Kit), a rapid and simple test that reliably detects nerve agents and pesticides (organophosphates, carbamates).

By using human acetylcholinesterase, M.A.I.K is highly sensitive to all substances that are toxic to humans and cause inhibition of AChE.